Comparison of methods for immunocytochemical detection of rotavirus infections.

Comparison of Enzyme Immunoassay, Immunochromatography, and RNA-Polyacrylamide-Gel Electrophoresis for Diagnosis of Rotavirus Infection in Children with Acute Gastroenteritis

Human rotavirus is a major etiologic agent for infantile diarrhea worldwide. It is responsible for up to 3.3 million deaths per year in children in developing countries. Various rapid and sensitive techniques have been developed to readily diagnose rotavirus gastroenteritis. In the present study, we compared the sensitivity and specificity of immunochromatography and RNA-polyacrylamide-gel elec...

Epidemiology of Rotavirus-Norovirus Co-Infection and Determination of Norovirus Genogrouping among Children with Acute Gastroenteritis in Tehran, Iran

Background: Enteric viruses, particularly human rotavirus and norovirus, have been shown to replace bacteria and parasites, as the most common pathogens responsible for acute diarrhea. However, there are still few epidemiological data on the simultaneous occurrence of these viruses in Iran. In this regard, the aim of this study was to assess the useful epidemiological data on the gastroenteriti...

Development of a Latex Agglutination Method for Diagnosis of Rotavirus Infections

Background: Rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. Since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included Electron Microscopy (EM) and enzyme immunoassay. These methods, however, are expensive and not readily available everywhere. We have developed a technique which can be used for ...

Development of Direct Sandwich ELISA test for Detection of Rotavirus in Clinical Specimen

Background and Aims: Rotaviruses are one of the main causative agents of acute viral gastroenteritis in both young human and animals. Several methods have been used for diagnosis of rotavirus infection. Most of these methods are not easily available in Iran. Methods: In our study attempt was made to develop a method which could replace the commercially imported Kit. Rota virus was purified and...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test